Glycosyl compounds
Research Products International Corp 4-Nitrophenyl-a-L-fucopyranoside, 100 Milligrams
Chromogenic substrate for detecting a-L-Fucosidase.
![Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 100 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71800-0.1.JPG-250.jpg)
Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 100 Milligrams
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
![Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 5 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71800-5.0.JPG-250.jpg)
Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 5 Grams
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
![Research Products International Corp Isopropyl-B-D-thio-galactopyranoside [IPTG], 5 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-I56000-5.03.JPG-250.jpg)
Research Products International Corp Isopropyl-B-D-thio-galactopyranoside [IPTG], 5 Grams
IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.
Dioxane-Free
Water Content: <0.49%
![Research Products International Corp Isopropyl-B-D-thio-galactopyranoside [IPTG], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-I56000-25.05.JPG-250.jpg)
Research Products International Corp Isopropyl-B-D-thio-galactopyranoside [IPTG], 25 Grams
IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.
Dioxane-Free
Water Content: <0.49%
Research Products International Corp Methyl-a-D-Mannopyranoside 25G
- CAS Number: 617-04-9
- Molecular Formula: C7H14O6
- Molecular Weight: 194.18
Research Products International Corp D-Gluc Acid Sod Salt Mono 25 G
- CAS Number: 207300-70-7
- Molecular Formula: C6H9NaO7 • H2O
- Molecular Weight: 234.14
Research Products International Corp D-Gluc Acid Sod Salt Mono 5G
- CAS Number: 207300-70-7
- Molecular Formula: C6H9NaO7 • H2O
- Molecular Weight: 234.14
Research Products International Corp D-Gluc Acid Sod Salt Mono 100G
- CAS Number: 207300-70-7
- Molecular Formula: C6H9NaO7 • H2O
- Molecular Weight: 234.14
Sigma Aldrich Fine Chemicals Biosciences Methyl cellulose viscosity: 15 cP | 9004-67-5 | MFCD00081763 | 100g
Methyl cellulose viscosity: 15 cP Purity: MW: | 9004-67-5 | MFCD00081763 | 100g
Sigma Aldrich Fine Chemicals Biosciences Methyl cellulose viscosity: 25 cP | 9004-67-5 | MFCD00081763 | 100g
Methyl cellulose viscosity: 25 cPMW: | 9004-67-5 | MFCD00081763 | 100g
Sigma Aldrich Fine Chemicals Biosciences 4-Methylumbelliferyl α-D-galactopyranoside | 38597-12-5 | MFCD00063278 | 100mg
4-Methylumbelliferyl α-D-galactopyranoside | Putity: ≥98% (TLC) | MW: 338.31 | 38597-12-5 | MFCD00063278 | 100mg